MD TP27:In vitro and in vivo validation of inhibition of different signaling pathways of immune complex-dependent ROS release

Neutrophil activation has been identified to contribute to blister formation in epidermolysis bullosa acquisita (EBA). In resumption to another MD-project (MD TP7) we now investigate about 36 compounds in order to validate the inhibition of immune complex-dependent neutrophil activation.

My aim is to find agents interrupting neutrophil signaling pathways and subsequently the activation of neutrophils. I want to decipher the signaling pathways leading to reactive oxygen species (ROS) release in neutrophils by using Western Blot. A local mouse model serves as an assay for the therapeutic effect and the tolerance of the drugs in vivo.